Research Briefing
Published: 11 September 2024
Nature Biotechnology
(2024)Cite this article
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Quadruplet codons allow multiplexing of non-canonical amino acids within single polypeptides in living cells. We show that including high-usage triplet codons after quadruplet codons can improve their decoding efficiency in genetic circuits, which allowed us to develop a system for the programmable biosynthesis of exotic macrocyclic peptides in cells.
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Fig. 1: Context effects of quadruplet decoding allow the biosynthesis of non-canonical macrocycles.
References
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This is a summary of: Costello, A. et al. Efficient genetic code expansion without host genome modifications. Nat. Biotechnol. https://doi.org/10.1038/s41587-024-02385-y (2024).
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Recoded gene circuits for multiplexed genetic code expansion.
Nat Biotechnol (2024). https://doi.org/10.1038/s41587-024-02387-w
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Published: 11 September 2024
DOI : https://doi.org/10.1038/s41587-024-02387-w
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